Using electron-microscopy images as a model for molecular replacement
- PMID: 11567152
- DOI: 10.1107/s0907444901013415
Using electron-microscopy images as a model for molecular replacement
Abstract
This review addresses the technical problems encountered while using models based on electron microscopy to generate initial phases for crystallographic studies. The test cases used were the gp6 portal protein with 13-fold rotational symmetry and the human hepatitis virus HepB, a viral assembly with T = 4 icosohedral symmetry.
Similar articles
-
Crystallographic analysis reveals the 12-fold symmetry of the bacteriophage phi29 connector particle.J Mol Biol. 1998 Aug 14;281(2):219-25. doi: 10.1006/jmbi.1998.1928. J Mol Biol. 1998. PMID: 9698542
-
Determination of the fold of the core protein of hepatitis B virus by electron cryomicroscopy.Nature. 1997 Mar 6;386(6620):88-91. doi: 10.1038/386088a0. Nature. 1997. PMID: 9052786
-
Shape and DNA packaging activity of bacteriophage SPP1 procapsid: protein components and interactions during assembly.J Mol Biol. 2000 Feb 11;296(1):117-32. doi: 10.1006/jmbi.1999.3450. J Mol Biol. 2000. PMID: 10656821
-
Breaking Symmetry in Viral Icosahedral Capsids as Seen through the Lenses of X-ray Crystallography and Cryo-Electron Microscopy.Viruses. 2018 Feb 7;10(2):67. doi: 10.3390/v10020067. Viruses. 2018. PMID: 29414851 Free PMC article. Review.
-
Molecular replacement--historical background.Acta Crystallogr D Biol Crystallogr. 2001 Oct;57(Pt 10):1360-6. doi: 10.1107/s0907444901009386. Epub 2001 Sep 21. Acta Crystallogr D Biol Crystallogr. 2001. PMID: 11567146 Review.
Cited by
-
X-ray structure determination using low-resolution electron microscopy maps for molecular replacement.Nat Protoc. 2015 Sep;10(9):1275-84. doi: 10.1038/nprot.2015.069. Epub 2015 Jul 30. Nat Protoc. 2015. PMID: 26226459 Free PMC article.
-
How cryo-electron microscopy and X-ray crystallography complement each other.Protein Sci. 2017 Jan;26(1):32-39. doi: 10.1002/pro.3022. Epub 2016 Sep 7. Protein Sci. 2017. PMID: 27543495 Free PMC article. Review.
-
Structure determination of a human virus by the combination of cryo-EM and X-ray crystallography.Biophys Rep. 2016;2(2):55-68. doi: 10.1007/s41048-016-0027-2. Epub 2016 Sep 2. Biophys Rep. 2016. PMID: 28018964 Free PMC article.
-
From lows to highs: using low-resolution models to phase X-ray data.Acta Crystallogr D Biol Crystallogr. 2013 Nov;69(Pt 11):2257-65. doi: 10.1107/S0907444913022336. Epub 2013 Oct 18. Acta Crystallogr D Biol Crystallogr. 2013. PMID: 24189238 Free PMC article. Review.
-
X-ray crystallography over the past decade for novel drug discovery - where are we heading next?Expert Opin Drug Discov. 2015;10(9):975-89. doi: 10.1517/17460441.2015.1061991. Epub 2015 Jul 15. Expert Opin Drug Discov. 2015. PMID: 26177814 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources