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. 2001 Oct;39(10):3709-11.
doi: 10.1128/JCM.39.10.3709-3711.2001.

Epidemiologic usefulness of spoligotyping for secondary typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110

W A Cronin  1 , J E Golub, L S Magder, N G Baruch, M J Lathan, L N Mukasa, N Hooper, J H Razeq, D Mulcahy, W H Benjamin, W R Bishai
Affiliations

Epidemiologic usefulness of spoligotyping for secondary typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110

W A Cronin et al. J Clin Microbiol. 2001 Oct.

Abstract

Restriction fragment length polymorphism (RFLP) analysis of IS6110 is commonly used to DNA fingerprint Mycobacterium tuberculosis. However, low-copy (< or =5) IS6110 M. tuberculosis strains are poorly differentiated, requiring secondary typing. When spoligotyping was used as the secondary method, only 13% of Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered strains had epidemiologic linkages to another patient, compared to 48% of those with high-copy strains clustered by IS6110 alone (P < 0.01). Spoligotyping did not improve a population-based molecular epidemiologic study of recent TB transmission.

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Figures

FIG. 1
FIG. 1
Distributions of M. tuberculosis patient isolates clustered by IS6110 alone and by IS6110 spoligotyping, by copy number, in Maryland 1996 to 1998. Note that secondary typing was conducted by the spoligotyping method for all isolates with ≤5 copies of IS6110 that were clustered by RFLP analysis of IS6110 alone.
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