Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Oct;140(1):99-105.
doi: 10.1128/jb.140.1.99-105.1979.

Restriction enzyme analysis of Bacillus subtilis ribosomal ribonucleic acid genes

C P Moran Jr, K F Bott

Restriction enzyme analysis of Bacillus subtilis ribosomal ribonucleic acid genes

C P Moran Jr et al. J Bacteriol. 1979 Oct.

Abstract

The organization of the ribosomal ribonucleic acid (rRNA) genes (rDNA) of Bacillus subtilis was examined by cleaving the genome with several restriction endonucleases. The rDNA sequences were assayed by hybridization with purified radioactive rRNA's. Our interpretation of the resulting electrophoretic patterns is strengthened by an analysis of a fragment of B. subtilis rDNA cloned in Escherichia coli. The results indicated that there are eight rRNA operons in B. subtilis. Each operon contains one copy of the sequences coding for 16S, 23S, and 5S rRNA. The sequences coding for 5S rRNA were shown to be more closely linked to the 23S rRNA genes than to the 16S rRNA genes.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. J Biol Chem. 1976 Jun 10;251(11):3480-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171-5 - PubMed
    1. FEBS Lett. 1977 Jul 1;79(1):77-9 - PubMed

Publication types

LinkOut - more resources