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. 1975 Sep 3;111(2):293-8.
doi: 10.1016/s0021-9673(00)99277-x.

Quantitative assay of diclofenac in biological material by gas-liquid chromatography

U P Geiger, P H Degen, A Sioufi

Quantitative assay of diclofenac in biological material by gas-liquid chromatography

U P Geiger et al. J Chromatogr. .

Abstract

Diclofenac and an internal standard are isolated from biological samples by acid-specific extraction and converted into the corresponding indolones with 0.5% sulphuric acid in 2,2,2-trifluorethanol. The neutral derivatives are isolated by solvent extraction and subjected to gas-liquid chromatography. The use of an electron capture detector permits the assay of diclofenac at levels down to 2 ng per sample.

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