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Review
. 2001 Oct 9;98(21):11837-8.
doi: 10.1073/pnas.221463598.

Navigating the human transcriptome

R L Strausberg  1 , G J Riggins
Affiliations
Review

Navigating the human transcriptome

R L Strausberg et al. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Figure 1
Figure 1
Idealized schematic view of ESTs generated by various public projects. A full-length cDNA is represented by the black bar, with nontranslated regions indicated by the stippled sections. The red arrows depict 5′ and 3′ ESTs based on the MGI approach; blue arrows depict the CGAP 3′ approach. Note that at both the 5′ and 3′ ends, alternate EST positions are possible based on transcript variations or incomplete cDNA synthesis. The purple arrow indicates the MGC EST strategy in which 5′ ESTs are generated to search for full-ORF clones. ORESTES tags are shown by the green arrows, which are spaced more evenly throughout the cDNA sequence. The green bars denote regions where sequence gaps exist that might be subject to the transcript-finishing approach of Camargo et al. The black arrow indicates where a SAGE tag might be located. This arrow is vertical to indicate that SAGE tags are located at a precise site within a transcript.
See this image and copyright information in PMC

Comment on

  • The contribution of 700,000 ORF sequence tags to the definition of the human transcriptome.
    Camargo AA, Samaia HP, Dias-Neto E, Simão DF, Migotto IA, Briones MR, Costa FF, Nagai MA, Verjovski-Almeida S, Zago MA, Andrade LE, Carrer H, El-Dorry HF, Espreafico EM, Habr-Gama A, Giannella-Neto D, Goldman GH, Gruber A, Hackel C, Kimura ET, Maciel RM, Marie SK, Martins EA, Nobrega MP, Paco-Larson ML, Pardini MI, Pereira GG, Pesquero JB, Rodrigues V, Rogatto SR, da Silva ID, Sogayar MC, Sonati MF, Tajara EH, Valentini SR, Alberto FL, Amaral ME, Aneas I, Arnaldi LA, de Assis AM, Bengtson MH, Bergamo NA, Bombonato V, de Camargo ME, Canevari RA, Carraro DM, Cerutti JM, Correa ML, Correa RF, Costa MC, Curcio C, Hokama PO, Ferreira AJ, Furuzawa GK, Gushiken T, Ho PL, Kimura E, Krieger JE, Leite LC, Majumder P, Marins M, Marques ER, Melo AS, Melo MB, Mestriner CA, Miracca EC, Miranda DC, Nascimento AL, Nobrega FG, Ojopi EP, Pandolfi JR, Pessoa LG, Prevedel AC, Rahal P, Rainho CA, Reis EM, Ribeiro ML, da Ros N, de Sa RG, Sales MM, Sant'anna SC, dos Santos ML, da Silva AM, da Silva NP, Silva WA Jr, da Silveira RA, Sousa JF, Stecconi D, Tsukumo F, Valente V, Soares F, Moreira ES, Nunes DN, Correa RG, Zalcberg H, Carvalho AF, Reis LF, Brentani RR, Simpson AJ, de Souza SJ. Camargo AA, et al. Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):12103-8. doi: 10.1073/pnas.201182798. Proc Natl Acad Sci U S A. 2001. PMID: 11593022 Free PMC article.

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    Camargo AA, Samaia HP, Dias-Neto E, Simão DF, Migotto IA, Briones MR, Costa FF, Nagai MA, Verjovski-Almeida S, Zago MA, Andrade LE, Carrer H, El-Dorry HF, Espreafico EM, Habr-Gama A, Giannella-Neto D, Goldman GH, Gruber A, Hackel C, Kimura ET, Maciel RM, Marie SK, Martins EA, Nobrega MP, Paco-Larson ML, Pardini MI, Pereira GG, Pesquero JB, Rodrigues V, Rogatto SR, da Silva ID, Sogayar MC, Sonati MF, Tajara EH, Valentini SR, Alberto FL, Amaral ME, Aneas I, Arnaldi LA, de Assis AM, Bengtson MH, Bergamo NA, Bombonato V, de Camargo ME, Canevari RA, Carraro DM, Cerutti JM, Correa ML, Correa RF, Costa MC, Curcio C, Hokama PO, Ferreira AJ, Furuzawa GK, Gushiken T, Ho PL, Kimura E, Krieger JE, Leite LC, Majumder P, Marins M, Marques ER, Melo AS, Melo MB, Mestriner CA, Miracca EC, Miranda DC, Nascimento AL, Nobrega FG, Ojopi EP, Pandolfi JR, Pessoa LG, Prevedel AC, Rahal P, Rainho CA, Reis EM, Ribeiro ML, da Ros N, de Sa RG, Sales MM, Sant'anna SC, dos Santos ML, da Silva AM, da Silva NP, Silva WA Jr, da Silveira RA, Sousa JF, Stecconi D, Tsukumo F, Valente V, Soares F, Moreira ES, Nunes DN, Correa RG, Zalcberg H, Carvalho AF, Reis LF, Brentani RR, Simpson AJ, de Souza SJ. Camargo AA, et al. Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):12103-8. doi: 10.1073/pnas.201182798. Proc Natl Acad Sci U S A. 2001. PMID: 11593022 Free PMC article.
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References

    1. Lander E S, Linton L M, Birren B, Nusbaum C, Zody M C, Baldwin J, Devon K, Dewar K, Doyle M, FitzHugh W, et al. Nature (London) 2001;409:860–921. - PubMed
    1. Venter J C, Adams M D, Myers E W, Li P W, Mural R J, Sutton G G, Smith H O, Yandell M, Evans C A, Holt R A, et al. Science. 2001;291:1304–1351. - PubMed
    1. Gong Q H, Cho J W, Huang T, Potter C, Gholami N, Basu N K, Kubota S, Carvalho S, Pennington M W, Owens I S, et al. Pharmacogenetics. 2001;11:357–368. - PubMed
    1. Yi X, White D M, Aisner D L, Baur J A, Wright W E, Shay J W. Neoplasia. 2000;2:433–440. - PMC - PubMed
    1. Edwalds-Gilbert G, Veraldi K L, Milcarek C. Nucleic Acids Res. 1997;25:2547–2561. - PMC - PubMed

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