Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Clinical Trial
. 2001 Aug;18(8):417-20.
doi: 10.1023/a:1016630603560.

Effects of growth hormone on in vitro maturation of germinal vesicle of human oocytes retrieved from small antral follicles

H A Hassan  1 , H Azab, A A Rahman, T M Nafee
Affiliations
Clinical Trial

Effects of growth hormone on in vitro maturation of germinal vesicle of human oocytes retrieved from small antral follicles

H A Hassan et al. J Assist Reprod Genet. 2001 Aug.

Abstract

Purpose: Evaluation of the effect of GH, administered in vivo, on in vitro maturation of human GV oocytes obtained from small follicles.

Methods: The study included 88 ICSI women stimulated with long agonist protocol. They were randomly allocated to two groups (Group 1, 44 women with 86 GV oocytes), were cotreated with GH (Group 2, no GH: 44 women with 67 GV oocytes). GV oocytes from small follicles were matured in vitro, injected, and their development observed.

Results: Resumption of meiosis was significantly higher in Group 1 compared to Group 2 (60 and 37.3% respectively). Significantly more M2 oocytes were obtained in Group 1 than in Group 2 (45.5 and 23.9% respectively). Fertilization rates were also significantly higher (51.9 and 18.8% respectively). No embryos were obtained in Group 2, whereas 21.1% of fertilized oocytes in Group 1 cleaved into class A embryos.

Conclusion: In vivo administration of GH enhanced in vitro maturation and fertilization of human GV oocytes retrieved from small antral follicles.

PubMed Disclaimer

References

    1. Bergh C, Broden H, Lundin K, Hamberger L. Comparison of fertilization, cleavage and pregnancy rates of oocytes from large and small follicles. Hum Reprod. 1998;13(7):1912–1915. - PubMed
    1. Gomez E, Tarin JJ, Pellicer A. Oocyte maturation in humans: The role of gonadotropins and growth factors. Fertil Steril. 1993;60(1):40–46. - PubMed
    1. Busacca M, Fusi FM, Brigante C, Bonzi V, Confiantini C, Vignali M, Ferrari A. Use of growth hormone releasing factor in ovulation induction in poor responders. J Reprod Med. 1996;41(9):699–703. - PubMed
    1. Dor J, Seidman DS, Amudai E, Bider D, Levran D, Mashiach S. Adjuvant growth hormone therapy in poor responders to in-vitro fertilization: A prospective randomized placebo-controlled double-blind study. Hum Reprod. 1995;10(1):40–43. - PubMed
    1. Homburg R, West C, Torresani T, Jacobs HS. A comparative study of single-dose growth hormone therapy as an adjuvant to gonadotrophin treatment for ovulation induction. Clin Endocrinol (Oxf) 1990;32(6):781–785. - PubMed

Publication types