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. 2001 Nov;45(11):3195-7.
doi: 10.1128/AAC.45.11.3195-3197.2001.

New model of oropharyngeal candidiasis in mice

Y Kamai  1 , M Kubota, Y Kamai, T Hosokawa, T Fukuoka, S G Filler
Affiliations

New model of oropharyngeal candidiasis in mice

Y Kamai et al. Antimicrob Agents Chemother. 2001 Nov.

Abstract

We established a straightforward murine model of oropharyngeal candidiasis. Mice were immunosuppressed with cortisone acetate, anesthetized, and then inoculated by placing cotton wool balls saturated with Candida albicans sublingually for 2 h. A prolonged, reproducible infection was induced. This model may be useful for antifungal screening or pathogenesis studies.

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Figures

FIG. 1
FIG. 1
Development of OPC in mice. (A) Effect of cortisone acetate and tetracycline on the numbers of viable C. albicans cells in the oral tissue on day 6. Data are the mean plus standard deviation for five mice per group. ∗∗∗, P < 0.001 by the Tukey test. CA, cortisone acetate; TET, tetracycline. (B) Time course of OPC in mice treated with cortisone acetate and tetracycline. Data are the mean ± standard deviation for five mice per group. Open circles, tongue; closed circles, nonlingual oral tissue; open triangles, esophagus.
FIG. 2
FIG. 2
White patches on the tongue of a mouse inoculated with C. albicans. The tongues of an uninfected (A) and infected (B) mouse were photographed on day 3. The arrow indicates the white patches on the tip of the tongue.
FIG. 3
FIG. 3
Histopathological analysis of the tongue (A) and the esophagus (B) tissue of mice infected with C. albicans for 4 days (PAS stain; bar = 50 μm).
FIG. 4
FIG. 4
The number of viable C. albicans cells in the oral tissue of mice that were treated with daily FLC at 10 mg/kg/dose starting on day 3. Results are the mean ± standard deviation for six mice per time point. Open circles, control mice; closed circles, mice treated with FLC.
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