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. 1975 Feb;28(1):1-9.
doi: 10.7883/yoken1952.28.1.

Studies on the potency test of antiserum for Weils disease by the intracutaneous method

K Akama, S Otani, M Mori, Y Arimitsu

Studies on the potency test of antiserum for Weils disease by the intracutaneous method

K Akama et al. Jpn J Med Sci Biol. 1975 Feb.

Abstract

A method for estimating the leptospiricidal activity of therapeutic antiserum for Weil's disease was improved by using the intracutaneous method in guinea pigs. The neutralization curves between the leptospiral suspensions for challenge and the antisera were shown to be linear over a wide range of the dosis of the pathogen. Although the reproducibility of the neutralization experiments was high, a significant divergence was observed among the slopes in various test samples. However, the trouble due to such a discrepancy in the slope may practically be reduced if such an antiserum preparation that has a regression coefficient close to the mean of those of various neutralization lines constructed by many different products is adopted as the reference. A practical method for the potency test was suggested.

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