Induction of soybean vegetative storage proteins and anthocyanins by low-level atmospheric methyl jasmonate

Abstract

Soybean seedlings were exposed to atmospheric methyl jasmonate (MJ) to determine if low levels of this compound could regulate the expression and accumulation of the vegetative storage proteins (VSPs) in soybeans. Low levels of atmospheric MJ induced the accumulation of three VSPs with molecular masses of 27 kDa, 29 kDa, and 94 kDa (vsp27, vsp29, and vsp94, respectively). Atmospheric MJ caused vsp94 to be accumulated in all above-ground organs of the seedling uniformly after just 3 days of exposure. vsp27 preferentially accumulated in shoot tips and primary leaves, whereas vsp29 preferentially accumulated in the cotyledons. In addition to these effects, MJ also induced the biosynthesis of anthocyanins in light-grown seedlings but inhibited anthocyanin biosynthesis in etiolated seedlings. It is concluded that low levels of atmospheric MJ regulate anthocyanin biosynthesis and the organspecific accumulation of VSPs in developing soybean seedlings. The organ-specific differential accumulation may reflect changes in the pattern of nitrogen partitioning between various compounds and/or organs. These results lend substance to the hypothesis that volatile MJ may act as a gaseous messenger or growth regulator in plants.