doi: 10.1093/nar/7.4.959.
The nucleotide sequence of human tRNAGly (anticodon GCC)
- PMID: 116197
- PMCID: PMC342274
- DOI: 10.1093/nar/7.4.959
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The nucleotide sequence of human tRNAGly (anticodon GCC)
Nucleic Acids Res.
.
Free PMC article
Abstract
The sequence of tRNAGCCGly from human placenta was determined by recently developed postlabeling techniques. The tRNA was digested completely with RNases T1 and A in the presence of alkaline phosphatase, the oligonucleotides were 3'-terminally (3H)-labeled, mapped on PEI-cellulose thin layers, isolated, and sequenced by methods based on base-specific cleavages. Overlaps were obtained by readout sequencing techniques on polyacrylamide gels and PEI-cellulose thin layers. The thin-layer readout technique was used also to locate and identify modified nucleotides. The primary structure was found to exhibit a large degree of homology (94.6%) with silkworm tRNAGCCGly but only 67.6% homology with human tRNACCCGly.
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