Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979;20(3):404-16.
doi: 10.1186/BF03546602.

DNases in milk and blood sera from different species

R Gudding

DNases in milk and blood sera from different species

R Gudding. Acta Vet Scand. 1979.

Abstract
in English, Norwegian

DNases were demonstrated in samples of colostrum and blood serum from man and various domestic animals. The measurable DNase activity recorded was highest in samples from cat and dog and lowest in samples from goat, horse, pig and sheep. In contrast to DNases produced by certain bacteria, these enzymes were thermo-labile and the activity was maximal in the area pH 5.0–5.5.

A modification of an agar medium originally described for the demonstration of bacterial DNases was found to be suitable for assays of DNases from colostrum, milk and serum.

DNaser ble påvist i prøver fra kolostrum og blodserum fra menneske og forskjellige husdyr. DNaseaktiviteten var høyest i prøver fra hund og katt og lavest i prøver fra geit, hest, gris og sau. I mot-setning til DNaser produsert av visse bakterier, var disse enzymene termolabile og aktiviteten var størst i pH-området 5,0 til 5,5.

En modifikasjon av et agarmedium beskrevet for påvisning av bakterielle DNaser ble funnet å være velegnet ved undersøkelse av DNaser fra kolostrum, melk og serum.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Bernandi, G.: Spleen acid deoxyribonuclease. In Boyer, P. D., ed.: The Enzymes 1971, 4, 271–287.
    1. Bernandi G, Griffé M. Studies on acid deoxyribonuclease. II. Isolation and characterization of spleen-acid deoxyribonuclease. Biochemistry. 1964;3:1419–1426. doi: 10.1021/bi00898a005. - DOI - PubMed
    1. Bernandi G, Bernandi A, Chersi A. Studies on acid hydrolases. I. A procedure for the preparation of acid deoxyribonuclease and other acid hydrolases. Biochim. biophys. Acta (Amst.) 1966;129:1–11. doi: 10.1016/0005-2787(66)90002-5. - DOI - PubMed
    1. Colombara E, Ihl M, Tagle M A. Serum deoxyribonuclease I and its inhibitor in growing and pregnant rats. J. Nutr. 1971;101:1525–1530. doi: 10.1093/jn/101.11.1525. - DOI - PubMed
    1. Cordonnier C, Bernandi G. A comparative study of acid deoxyribo-nucleases extracted from different tissues and species. Canad. J. Biochem. 1968;46:989–995. doi: 10.1139/o68-148. - DOI - PubMed

Substances

LinkOut - more resources