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. 1979;20(3):404-16.
doi: 10.1186/BF03546602.

DNases in milk and blood sera from different species

R Gudding

DNases in milk and blood sera from different species

R Gudding. Acta Vet Scand. 1979.

Abstract
in English, Norwegian

DNases were demonstrated in samples of colostrum and blood serum from man and various domestic animals. The measurable DNase activity recorded was highest in samples from cat and dog and lowest in samples from goat, horse, pig and sheep. In contrast to DNases produced by certain bacteria, these enzymes were thermo-labile and the activity was maximal in the area pH 5.0–5.5.

A modification of an agar medium originally described for the demonstration of bacterial DNases was found to be suitable for assays of DNases from colostrum, milk and serum.

DNaser ble påvist i prøver fra kolostrum og blodserum fra menneske og forskjellige husdyr. DNaseaktiviteten var høyest i prøver fra hund og katt og lavest i prøver fra geit, hest, gris og sau. I mot-setning til DNaser produsert av visse bakterier, var disse enzymene termolabile og aktiviteten var størst i pH-området 5,0 til 5,5.

En modifikasjon av et agarmedium beskrevet for påvisning av bakterielle DNaser ble funnet å være velegnet ved undersøkelse av DNaser fra kolostrum, melk og serum.

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