Alteration of integrins by interleukin-1alpha in human pancreatic cancer cells
- PMID: 11668210
- DOI: 10.1097/00006676-200111000-00011
Alteration of integrins by interleukin-1alpha in human pancreatic cancer cells
Abstract
Introduction: Adhesion of tumor cells to extracellular matrix (ECM) proteins plays an important role in tumor invasion and metastasis.
Aims: To investigate the expression of integrins in human pancreatic cancer cell lines and its alteration by interleukin (IL)-1alpha to examine the mechanism of adhesion of metastatic human pancreatic cancer cells to ECM proteins.
Methodology: The expression of integrin subunits and their alteration by IL-1alpha were examined by flow-cytometric analysis and cellular enzyme-linked immunosorbent assay in three metastatic human pancreatic cancer cell lines (AsPC-1, BxPC-3, and SW1990) and two nonmetastatic cancer cell lines (PaCa-2 and PANC-1). In addition, assays of cancer cell adhesion to ECM proteins were performed to investigate if increased integrin expression actually affected the adhesive interaction between cancer cells and the putative integrin ECM ligands.
Results: The alpha(6) subunit expressed in metastatic cancer cells was enhanced by IL-1alpha. Metastatic cancer cells also showed preferential adherence to laminin compared with nonmetastatic cancer cells, and this was enhanced by IL-1alpha.
Conclusion: In pancreatic cancer, the enhancement of alpha(6)beta(1) integrin by IL-1alpha through IL-1 receptor type I, as well as the expression of alpha(6)beta(1) integrin, plays an important role in metastasis formation.
Similar articles
-
Interleukin-1alpha enhances integrin alpha(6)beta(1) expression and metastatic capability of human pancreatic cancer.Oncology. 2003;65(2):167-73. doi: 10.1159/000072343. Oncology. 2003. PMID: 12931024
-
Enhancement of integrins by interleukin-1alpha, and their relationship with metastatic and invasive behavior of human pancreatic ductal adenocarcinoma cells.J Surg Oncol. 2003 Jan;82(1):51-6. doi: 10.1002/jso.10187. J Surg Oncol. 2003. PMID: 12501168
-
Interleukin-1alpha enhances the aggressive behavior of pancreatic cancer cells by regulating the alpha6beta1-integrin and urokinase plasminogen activator receptor expression.BMC Cell Biol. 2006 Feb 20;7:8. doi: 10.1186/1471-2121-7-8. BMC Cell Biol. 2006. PMID: 16504015 Free PMC article.
-
The characteristics and the multiple functions of integrin β1 in human cancers.J Transl Med. 2023 Nov 6;21(1):787. doi: 10.1186/s12967-023-04696-1. J Transl Med. 2023. PMID: 37932738 Free PMC article. Review.
-
Integrin clipping: a novel adhesion switch?J Cell Biochem. 2004 Jan 1;91(1):26-35. doi: 10.1002/jcb.10675. J Cell Biochem. 2004. PMID: 14689578 Free PMC article. Review.
Cited by
-
Expression and prognostic roles of integrins and interleukin-1 receptor type I in patients with ductal adenocarcinoma of the pancreas.Dig Dis Sci. 2003 Jul;48(7):1241-50. doi: 10.1023/a:1024276821731. Dig Dis Sci. 2003. PMID: 12870779
-
Type I collagen and divalent cation shifts disrupt cell-cell adhesion, increase migration, and decrease PTHrP, IL-6, and IL-8 expression in pancreatic cancer cells.Int J Gastrointest Cancer. 2005;36(3):131-46. doi: 10.1385/IJGC:36:3:131. Int J Gastrointest Cancer. 2005. PMID: 16720909
-
The alpha2beta1 integrin mediates the malignant phenotype on type I collagen in pancreatic cancer cell lines.Br J Cancer. 2006 May 8;94(9):1311-9. doi: 10.1038/sj.bjc.6603088. Br J Cancer. 2006. PMID: 16622460 Free PMC article.
-
Engineering of biomaterials for tumor modeling.Mater Today Adv. 2020 Dec;8:100117. doi: 10.1016/j.mtadv.2020.100117. Epub 2020 Nov 12. Mater Today Adv. 2020. PMID: 34541484 Free PMC article.
-
Predictive Value of MUC5AC Signature in Pancreatic Ductal Adenocarcinoma: A Hypothesis Based on Preclinical Evidence.Int J Mol Sci. 2023 Apr 30;24(9):8087. doi: 10.3390/ijms24098087. Int J Mol Sci. 2023. PMID: 37175794 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
