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Comparative Study
. 1975 Jan;114(1 Pt 1):51-4.

Quantitative determination of allotypic and idiotypic markers with antisera coupled to N-hydroxysuccinimide-activated sepharose

A B Gottlieb, R K Seide, T J Kindt
  • PMID: 1167562
Comparative Study

Quantitative determination of allotypic and idiotypic markers with antisera coupled to N-hydroxysuccinimide-activated sepharose

A B Gottlieb et al. J Immunol. 1975 Jan.

Abstract

N-hydroxysuccinimide activated Sepharose beads (HAS) were used to immobilize allotypic and idiotypic antisera for use in radioimmunoassays. These HAS antisera were compared to antisera solidified with ethylchloroformate (ECF) with respect to their reactivity, specificity, and ability to detect subspecificities of the group a allotypes. The HAS antisera rated favorably in all comparisons with ECF antisera and are far superior with respect to ease of preparation and retention of antibody activity.

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