doi: 10.1007/BF02408073.
The occurrence of chondroitin-6-sulphate in adult human compact bone tissue
- PMID: 116757
- DOI: 10.1007/BF02408073
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The occurrence of chondroitin-6-sulphate in adult human compact bone tissue
Calcif Tissue Int.
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Abstract
Compact bone tissue chondroitin sulphate, previously considered to be pure chondroitin-4-sulphate, was isolated from three adult human femoral diaphyses and digested with chondroitinase ABC. Assaying these digests by means of high performance liquid chromatography we could demonstrate that 12 to 14% of the disaccharide residues were 6-sulphated. The 4/6-ratios were also studied in chondroitin sulphate fractions of different molecular size. Slightly increasing amounts of 6-sulphated disaccharides were found with increasing size of the chondroitin sulphates.
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