Microbiological assay for determination of ofloxacin injection

Abstract

A simple, sensitive and specific agar diffusion bioassay for the antibacterial ofloxacin was developed using a strain of Micrococcus luteus ATCC 9341 as the test organism, ofloxacin at concentration ranging 12-27 microg ml(-1) could be measured in injection. A prospective validation of the method showed that method was linear (r=0.9994) and precise (CV=1.14). UV spectrophotometric and high performance liquid chromatographic techniques were chosen as a comparison methods for the determination of ofloxacin. The results obtained by three methods were statistically analyzed by analysis of variance (ANOVA) and the results obtained indicate that there is no significant difference among these methods.