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. 1975 Feb;148(2):472-5.
doi: 10.3181/00379727-148-38564.

Dengue virus plaque formation on microplate cultures and its application to virus neutralization (38564)

N Fujita, M Tamura, S Hotta

Dengue virus plaque formation on microplate cultures and its application to virus neutralization (38564)

N Fujita et al. Proc Soc Exp Biol Med. 1975 Feb.

Abstract

Dengue virus plaque formation on BHK-21 cell microplate cultures was described. The clear plaques were visible usually 5 days after incubation in a CO-2 incubator at 37 degrees. The cells cultured in a 3-oz bottle were sufficient to prepare two microculture plates which were usually ready for use after 1-2 days of cultivation in the CO-2 incubator at 37 degrees. The overall procedures were easy and of economic advantage.

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