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Comparative Study
. 2001 Nov;8(6):1282-5.
doi: 10.1128/CDLI.8.6.1282-1285.2001.

Efficacy of a less-sensitive enzyme immunoassay (3A11-LS) for early diagnosis of human immunodeficiency virus Type 1 infection in infants

D Candal  1 , M Bulterys, E J Abrams, R W Steketee, B S Parekh
Affiliations
Comparative Study

Efficacy of a less-sensitive enzyme immunoassay (3A11-LS) for early diagnosis of human immunodeficiency virus Type 1 infection in infants

D Candal et al. Clin Diagn Lab Immunol. 2001 Nov.

Abstract

We evaluated a less-sensitive enzyme immunoassay (3A11-LS) for its possible use for early diagnosis of human immunodeficiency virus type 1 (HIV-1) infection in infants. The results were compared with those from the immunoglobulin G-capture enzyme immunoassay. A total of 239 sera from 77 infants were tested. All 25 sera from the 10 infants born to seronegative mothers were found to be negative by both assays. Forty-one seroreverting infants showed a complete decay of maternal antibodies by 4 months by the 3A11-LS assay. However, the assay detected HIV antibodies in only 9 (36%) of 25 sera collected from infected infants between 4 and 6 months and in 27 (63%) of 43 sera collected after 6 months of age. Further analysis with alternative cutoff values indicated that the 3A11-LS had a sensitivity of 12 to 44% and a specificity of 90 to 100% for infants between 4-6 months of age. This data suggest that a diagnosis of HIV infection in some of the infants could be made after 4 months of age by the 3A11-LS assay, although a negative 3A11-LS test result may not rule out infection and may require a further followup.

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Figures

FIG. 1
FIG. 1
HIV-1 antibody detected in seroreverting infants (A, upper panels) or in HIV-1-infected infants (B, lower panels) with respect to their ages. SOD values obtained by the 3A11-LS assay (●, left panels) or OD values by IgG-CEIA (○, right panels) are shown.
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