Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000;18(7):589-97.
doi: 10.1023/a:1011927004307.

Migratory activity of human glioma cell lines in vitro assessed by continuous single cell observation

T Demuth  1 , N J Hopf, O Kempski, D Sauner, M Herr, A Giese, A Perneczky
Affiliations

Migratory activity of human glioma cell lines in vitro assessed by continuous single cell observation

T Demuth et al. Clin Exp Metastasis. 2000.

Abstract

A new migration assay, the time-lapse individual cell migration assay (TIM-assay), was developed, which allows the observation of cells over 24 h under controlled conditions. Using this technique, the migratory behavior of 8 human glioblastoma cell lines in vitro was studied. Special features are simultaneous documentation of migratory parameters of individual cells, i.e., migration velocities and migration paths of individual cells. Migration velocity for cell populations of the same cell line ranged from 0 to 24 microm/h. The migration paths were examined for being directional. Two thirds of all cells showed directional migration. Migration paths were further classified according to visual judgements for being linear, oscillating or mixed. The migration index had a mean of 91%. The presented TIM-assay allows the assessment of several new parameters. that may be useful to identify subgroups of gliomas with different biological characteristics.

PubMed Disclaimer

References

    1. Neurosurgery. 1991 May;28(5):652-8 - PubMed
    1. J Neurosurg. 1995 Apr;82(4):615-22 - PubMed
    1. Int J Cancer. 1995 Sep 15;62(6):767-71 - PubMed
    1. Brain Pathol. 1994 Apr;4(2):167-79 - PubMed
    1. Cancer Res. 1994 Jul 15;54(14):3897-904 - PubMed

Publication types