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Review
. 2001 Sep;19(1):29-44.
doi: 10.1385/MB:19:1:029.

Strategies for signal amplification in nucleic acid detection

S C Andras  1 , J B Power, E C Cocking, M R Davey
Affiliations
Review

Strategies for signal amplification in nucleic acid detection

S C Andras et al. Mol Biotechnol. 2001 Sep.

Abstract

Many aspects of molecular genetics necessitate the detection of nucleic acid sequences. Current approaches involving target amplification (in situ PCR, Primed in situ Labeling, Self-Sustained Sequence Replication, Strand Displacement Amplification), probe amplification (Ligase Chain Reaction, Padlock Probes, Rolling Circle Amplification) and signal amplification (Tyramide Signal Amplification, Branched DNA Amplification) are summarized in the present review, together with their advantages and limitations.

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