Review
doi: 10.1146/annurev.bioeng.2.1.339.
Antibody engineering
Affiliations
- PMID: 11701516
- DOI: 10.1146/annurev.bioeng.2.1.339
Item in Clipboard
Review
Antibody engineering
Annu Rev Biomed Eng.
2000.
Abstract
Antibodies are unique in their high affinity and specificity for a binding partner, a quality that has made them one of the most useful molecules for biotechnology and biomedical applications. The field of antibody engineering has changed rapidly in the past 10 years, fueled by novel technologies for the in vitro isolation of antibodies from combinatorial libraries and their functional expression in bacteria. This review presents an overview of the methods available for the de novo generation of human antibodies, for engineering antibodies with increased antigen affinity, and for the production of antibody fragments. Select applications of recombinant antibodies are also presented.
Similar articles
-
Generation of antibody molecules through antibody engineering.Methods Mol Biol. 2003;207:3-25. doi: 10.1385/1-59259-334-8:03. Methods Mol Biol. 2003. PMID: 12412465 Review. No abstract available.
-
Directed evolution of proteins for increased stability and expression using yeast display.Arch Biochem Biophys. 2012 Oct 15;526(2):174-80. doi: 10.1016/j.abb.2012.04.022. Epub 2012 May 3. Arch Biochem Biophys. 2012. PMID: 22575387 Review.
-
Bacterial display in combinatorial protein engineering.Biotechnol J. 2011 Sep;6(9):1115-29. doi: 10.1002/biot.201100129. Epub 2011 Jul 22. Biotechnol J. 2011. PMID: 21786423 Review.
-
Antibody evolution beyond Nature.Nat Biotechnol. 2002 Dec;20(12):1189-90. doi: 10.1038/nbt1202-1189. Nat Biotechnol. 2002. PMID: 12454662 No abstract available.
-
Protein engineering of antibodies.Crit Rev Biotechnol. 1992;12(5-6):437-62. doi: 10.3109/07388559209114235. Crit Rev Biotechnol. 1992. PMID: 1423650 Review.
Cited by
-
Design, expression, and stability of a diverse protein library based on the human fibronectin type III domain.Protein Sci. 2007 Mar;16(3):476-84. doi: 10.1110/ps.062498407. Protein Sci. 2007. PMID: 17322532 Free PMC article.
-
Generation of high-affinity chicken single-chain Fv antibody fragments for measurement of the Pseudonitzschia pungens toxin domoic acid.Appl Environ Microbiol. 2006 May;72(5):3343-9. doi: 10.1128/AEM.72.5.3343-3349.2006. Appl Environ Microbiol. 2006. PMID: 16672476 Free PMC article.
-
mRNA display design of fibronectin-based intrabodies that detect and inhibit severe acute respiratory syndrome coronavirus nucleocapsid protein.J Biol Chem. 2009 Jun 26;284(26):17512-20. doi: 10.1074/jbc.M901547200. Epub 2009 Apr 13. J Biol Chem. 2009. PMID: 19364769 Free PMC article.
-
Single Chain Antibody Fragment against Venom from the Snake Daboia russelii formosensis.Toxins (Basel). 2017 Oct 27;9(11):347. doi: 10.3390/toxins9110347. Toxins (Basel). 2017. PMID: 29076991 Free PMC article.
-
Self-assembly of antibodies by chemical induction.Angew Chem Int Ed Engl. 2008;47(52):10179-82. doi: 10.1002/anie.200803507. Angew Chem Int Ed Engl. 2008. PMID: 19025747 Free PMC article. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
