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. 2001 Sep:945:289-91.
doi: 10.1111/j.1749-6632.2001.tb03897.x.

Possible origin of extrachromosomal DNA from human lymphocytes following in vitro treatment by phytohemagglutinin

J S Chen  1 , M G Chen
Affiliations

Possible origin of extrachromosomal DNA from human lymphocytes following in vitro treatment by phytohemagglutinin

J S Chen et al. Ann N Y Acad Sci. 2001 Sep.

Abstract

Lymphocytes respond to chemical or pathological challenges by synthesizing episomes or extrachromosomal DNA (eDNA). Phytohemagglutinin (PHA) is a mitogen for T lymphocytes and can stimulate the increased production of episomes as well as the release of eDNA, interleukin-2, and other factors. However, the origin of this eDNA is largely unknown. To confirm the observation on the localization of an antibody directed against a 23-kb segment of bull acrosomal DNA in cultured human lymphocytes, we performed analyses by fluorescence in situ hybridization (FISH) using the same DNA as a molecular probe.

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