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. 1975 Mar;92(3):305-9.
doi: 10.1111/j.1365-2133.1975.tb03080.x.

Primary cell culture for biochemical studies of human keratinocytes. A method for production of very large numbers of cells without the necessity of subculturing techniques

B A Flaxman, R A Harper

Primary cell culture for biochemical studies of human keratinocytes. A method for production of very large numbers of cells without the necessity of subculturing techniques

B A Flaxman et al. Br J Dermatol. 1975 Mar.

Abstract

Starting with large numbers of small split-thickness explants of human skin, it is possible to grow ample numbers of keratinocytes in vitro as primary cell cultures to permit biochemical studies without the need of subculturing techniques.

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