Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 Dec;45(12):3625-8.
doi: 10.1128/AAC.45.12.3625-3628.2001.

Nonculture prediction of Neisseria meningitidis susceptibility to penicillin

A Antignac  1 , J M Alonso, M K Taha
Affiliations

Nonculture prediction of Neisseria meningitidis susceptibility to penicillin

A Antignac et al. Antimicrob Agents Chemother. 2001 Dec.

Abstract

We developed a nonculture method to predict the susceptibility of Neisseria meningitidis to penicillin G. The penA gene was amplified and submitted to restriction fragment length polymorphism analysis. This approach was first validated with a collection of 75 meningococcal strains of known phenotypes. It was next successfully applied to 29 clinical samples.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Agarose gel electrophoresis of PCR-amplified DNA fragment of penA gene (A) and corresponding restriction fragment length patterns after digestion by TaqI (B) of Pens and Peni N. meningitidis strains (one representative strain by RFLP).
See this image and copyright information in PMC

References

    1. Antignac A, Kriz P, Tzanakaki G, Alonso J-M, Taha M-K. Polymorphism of Neisseria meningitidis penA gene associated with reduced susceptibility to penicillin. J Antimicrob Chemother. 2001;47:285–296. - PubMed
    1. Arreaza L, Vazquez J A. Abstracts of the 6th European Monitoring Group on Meningococci. 2001. Proposal for standardization of media and methods to be used on the determination of the susceptibility level of resistance to antibiotics in Neisseria meningitidis, pp. 40–43. Orebro, Sweden.
    1. Borrow R, Claus H, Guiver M, Smart L, Jones D M, Kaczmarski E B, Frosch M, Fox A J. Non-culture diagnosis and serogroup determination of meningococcal B and C infection by a sialyltransferase (siaD) PCR ELISA. Epidemiol Infect. 1997;118:111–117. - PMC - PubMed
    1. Borrow R, Claus H, Chaudhry U, Guiver M, Kaczmarski E B, Frosch M, Fox A J. siaD PCR ELISA for confirmation and identification of serogroup Y and W135 meningococcal infections. FEMS Microbiol Lett. 1998;159:209–214. - PubMed
    1. Bowler L D, Zhang Q-Y, Riou J-Y, Spratt B G. Interspecies recombination between the penA genes of Neisseria meningitidis and commensal Neisseria species during the emergence of penicillin resistance in N. meningitidis: natural events and laboratory simulation. J Bacteriol. 1994;176:333–337. - PMC - PubMed

Publication types

MeSH terms

LinkOut - more resources