Osteoclast recruitment to sites of compression in orthodontic tooth movement

Abstract

Although it is widely acknowledged that osteoclasts are formed by the fusion of mononuclear cells of hematopoietic origin, it has been extremely difficult to understand how they originate after appliance activation. The purpose of this study was to quantify osteoclast recruitment at compression sites as a function of time following orthodontic force application. Appliances were placed in 96 rats. At day 0, the animals were randomized to either appliance activation or sham activation followed by the injection of 5-bromo-2'-deoxyuridine (BrdU). Thus, BrdU was incorporated into the nuclei of cells in S-phase, including hematopoietic stem cells. Groups of 10 to 13 rats were killed at 1, 3, 5, and 7 days after activation/sham, and the tissue samples were prepared. The numbers of BrdU-labeled cells positively stained with tartrate-resistant acid phosphatase (TRAP) were measured in the periodontium. A significant number of BrdU-positive preosteoclasts was observed in the periodontal ligament (PDL) and bone surface at day 3. The number of osteoclastic cells in the bone marrow also peaked at day 3; however, the highest percentage of cells in this location was observed at day 1. These data suggest that osteoclasts in the PDL originate by the fusion of recently recruited preosteoclasts from the marrow instead of from local PDL cells. Furthermore, the alveolar bone marrow plays a role in the formation of osteoclasts during orthodontic tooth movement.