[The relationship between IgA nephropathy and HBV infection]

Abstract

Objective: To clarify the correlation between hepatitis B virus (HBV) infection and the pathogenesis of IgA nephropathy.

Methods: Ninety-one cases of primary IgA nephropathy were investigated by using in situ hybridization(HBV DNA), immunohistochemistry (HBAg) and HBV DNA-HBAg double staining techniques. In some of the cases, Southern blot analysis and serologic HBV infectious markers were detected also.

Results: The positive rate of HBAg in renal tissue detected by immunohistochemistry was 69% (63/91) and that of the HBV DNA detected by in situ hybridization was 43% (39/91). Eighteen of 27 cases showed HBV DNA positive in Southern blot analysis, and all of the positive cases were identified to be the integrated form. Among them, 17 cases were HBAg detectable in renal tissue by immunohistochemistry and 16 cases were HBV DNA detectable by in situ hybridization simultaneously. The positive rate of the expression of HBcAg [77% (30/39)] and HBsAg [72% (28/39)] of the tubular epithelial cells with HBV DNA signals in nuclears by in situ hybridization was significantly higher than that of the cells without HBV DNA signals(10%, 21%). The double staining showed that most of the HBV DNA positive tubular epithelial cells coexpressed HBsAg and(or) HBcAg.

Conclusion: The renal tissue of some of IgA nephropathy infected with HBV directly and express HBAg in situ.