Axo-axonic GABA-immunopositive synapses on the primary afferent fibers in frogs

Abstract

In three frog species Rana esculenta, Rana temporaria and Xenopus laevis, the contacts established by gamma-aminobutyric acid and glutamate decarboxylase immunoreactive (-ir) terminals upon primary afferent fibers were studied using confocal and electron microscopy. For confocal microscopy, the primary afferent fibers were labeled through the dorsal root with Dextran-Texas Red, whereas gamma-aminobutyric acid and glutamate decarboxylase immunoreactivity were revealed with fluorescein isothiocyanate. Appositions of gamma-aminobutyric acid and glutamate decarboxylase immunoreactive profiles onto primary afferent fibers were observed and were considered as putative axo-axonic contacts of GABAergic terminals upon primary afferents. The latter was confirmed by the ultrastructural finding of axo-axonic synapses from gamma-aminobutyric acid immunopositive boutons upon the HRP-labeled primary afferent fibers in postembedding immunoelectron microscopic study. Such synapses may represent the morphological basis of GABAergic presynaptic inhibition of primary afferent fibers.