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. 2001 Dec;39(12):4349-56.
doi: 10.1128/JCM.39.12.4349-4356.2001.

Staphylococcus aureus isolated in cases of impetigo produces both epidermolysin A or B and LukE-LukD in 78% of 131 retrospective and prospective cases

A Gravet  1 , P Couppié, O Meunier, E Clyti, B Moreau, R Pradinaud, H Monteil, G Prévost
Affiliations

Staphylococcus aureus isolated in cases of impetigo produces both epidermolysin A or B and LukE-LukD in 78% of 131 retrospective and prospective cases

A Gravet et al. J Clin Microbiol. 2001 Dec.

Abstract

Clinical symptoms of impetigo and staphylococcal scalded skin syndrome may not only be expressed as the splitting of cell layers within the epidermis but are often accompanied by some localized inflammation. Toxin patterns of Staphylococcus aureus isolates originating from patients with impetigo and also from those with other primary and secondary skin infections in a retrospective isolate collection in France and a prospective isolate collection in French Guiana revealed a significant association (75% of the cases studied) of impetigo with production of at least one of the epidermolysins A and B and the bicomponent leucotoxin LukE-LukD (P < 0.001). However, most of the isolates were able to produce one of the nonubiquitous enterotoxins. Pulsed-field gel electrophoresis (PFGE) of genomic DNA hydrolyzed with SmaI showed a polymorphism of the two groups of isolates despite the fact that endemic clones were suspected in French Guiana and France. The combination of toxin patterns with PFGE fingerprinting may provide further discrimination among isolates defined in a given cluster or a given pulsotype and account for a specific virulence. The new association of toxins with a clinical syndrome may reveal principles of the pathological process.

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Figures

FIG. 1
FIG. 1
Three PFGE gels of 24 independent S. aureus isolates from patients with impetigo with correspondences of pulsotypes (bottom) in both retrospective and prospective series; schematics of the pulsotypes are shown in Fig. 2 and 3. Ladders (L) were obtained from statistic oligomers of bacteriophage lambda DNA (New England Biolabs).
FIG. 2
FIG. 2
Schematic representation and computer-aided dendrogram analysis of the 83 SmaI pulsotypes of S. aureus DNA from strains producing at least one epidermolysin and isolated from patients with impetigo in metropolitan France. Columns: a, pulsotype numbers; b, number of isolates corresponding to each pulsotype (numbers in parentheses are the numbers of isolates producing at least one epidermolysin and LukE-LukD); c, different phage groups, with sensitivities of isolates distinguished in each pulsotype (NC, nonclassified phages 81, 94, 95, and 96; NT, nontypeable isolate).
FIG. 3
FIG. 3
Schematic representation and computer-aided dendrogram analysis of the 48 SmaI pulsotypes of S. aureus DNA from strains originating from patients with impetigo in French Guiana. Columns: a, pulsotype numbers; b, number of isolates corresponding to each pulsotype (numbers in parentheses are the numbers of isolates producing at least one epidermolysin and LukE-LukD); c, sensitivity of isolates distinguished in each pulsotype to phages belonging to phage groups (NC, nonclassified phages 81, 94, 95, and 96).
See this image and copyright information in PMC

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