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. 2001 Dec;39(12):4518-9.
doi: 10.1128/JCM.39.12.4518-4519.2001.

Automation of fluorescence-based PCR for confirmation of meningococcal disease

M A Diggle  1 , G F Edwards, S C Clarke
Affiliations

Automation of fluorescence-based PCR for confirmation of meningococcal disease

M A Diggle et al. J Clin Microbiol. 2001 Dec.

Abstract

A fluorescence-based PCR method was developed, fully automated, and used to confirm infection with Neisseria meningitidis by detection of the meningococcus-specific ctrA gene. The method provided a highly sensitive, high-throughput assay that was reproducible and less labor-intensive than manual methods.

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References

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