ERM-dependent movement of CD43 defines a novel protein complex distal to the immunological synapse
- PMID: 11728336
- DOI: 10.1016/s1074-7613(01)00224-2
ERM-dependent movement of CD43 defines a novel protein complex distal to the immunological synapse
Abstract
The large mucin CD43 is actively excluded from T cell/APC interaction sites, concentrating in a membrane domain distal to the site of TCR engagement. The cytoplasmic region of CD43 was necessary and sufficient for this antipodal movement. ERM cytoskeletal adaptor proteins colocalized with CD43 in this domain. An ERM dominant-negative mutant blocked the distal accumulation of CD43 and another known ERM binding protein, Rho-GDI. Inhibition of ERM function decreased the production of IL-2 and IFNgamma, without affecting PKC(theta) focusing or CD69 upregulation. These results indicate that ERM proteins organize a complex distal to the T cell/APC interaction site and provide evidence that full T cell activation may involve removal of inhibitory proteins from the immunological synapse.
Similar articles
-
The distal pole complex: a novel membrane domain distal to the immunological synapse.Immunol Rev. 2002 Nov;189:111-22. doi: 10.1034/j.1600-065x.2002.18910.x. Immunol Rev. 2002. PMID: 12445269 Review.
-
FERMing up the synapse.Immunity. 2001 Nov;15(5):683-6. doi: 10.1016/s1074-7613(01)00237-0. Immunity. 2001. PMID: 11728330 Review.
-
CD43 regulation of T cell activation is not through steric inhibition of T cell-APC interactions but through an intracellular mechanism.J Exp Med. 2004 May 3;199(9):1277-83. doi: 10.1084/jem.20021602. Epub 2004 Apr 26. J Exp Med. 2004. PMID: 15117976 Free PMC article.
-
Exclusion of CD43 from the immunological synapse is mediated by phosphorylation-regulated relocation of the cytoskeletal adaptor moesin.Immunity. 2001 Nov;15(5):691-701. doi: 10.1016/s1074-7613(01)00231-x. Immunity. 2001. PMID: 11728332
-
Polar redistribution of the sialoglycoprotein CD43: implications for T cell function.J Immunol. 2002 Apr 15;168(8):3740-6. doi: 10.4049/jimmunol.168.8.3740. J Immunol. 2002. PMID: 11937524
Cited by
-
Structural basis of adhesion-molecule recognition by ERM proteins revealed by the crystal structure of the radixin-ICAM-2 complex.EMBO J. 2003 Feb 3;22(3):502-14. doi: 10.1093/emboj/cdg039. EMBO J. 2003. PMID: 12554651 Free PMC article.
-
Mechanism of isorhynchophylline in lipopolysaccharide-induced acute lung injury based on proteomic technology.Front Pharmacol. 2024 May 30;15:1397498. doi: 10.3389/fphar.2024.1397498. eCollection 2024. Front Pharmacol. 2024. PMID: 38873411 Free PMC article.
-
The Biological Significance of Trogocytosis.Results Probl Cell Differ. 2024;73:87-129. doi: 10.1007/978-3-031-62036-2_5. Results Probl Cell Differ. 2024. PMID: 39242376 Free PMC article.
-
Regulation of actin cytoskeletal dynamics in T cell development and function.Front Immunol. 2025 Jun 10;16:1622928. doi: 10.3389/fimmu.2025.1622928. eCollection 2025. Front Immunol. 2025. PMID: 40557146 Free PMC article. Review.
-
CD43 signals induce Type One lineage commitment of human CD4+ T cells.BMC Immunol. 2007 Nov 23;8:30. doi: 10.1186/1471-2172-8-30. BMC Immunol. 2007. PMID: 18036228 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases