Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 Dec;33(6):461-6.
doi: 10.1046/j.1472-765x.2001.01032.x.

Detection, distribution and selection of microsatellites (SSRs) in the genome of the yeast Saccharomyces cerevisiae as molecular markers

M A Pérez  1 , F J Gallego, I Martínez, P Hidalgo
Affiliations

Detection, distribution and selection of microsatellites (SSRs) in the genome of the yeast Saccharomyces cerevisiae as molecular markers

M A Pérez et al. Lett Appl Microbiol. 2001 Dec.

Abstract

Aims: The aim of this work was the selection of six polymorphic microsatellite loci for their use as molecular markers in the identification, typification and genetic differentiation of S. cerevisiae strains.

Methods and results: The selection was undertaken following a search of the genomic DNA database of Saccharomyces cerevisiae for simple tandem repeat sequences (microsatellites) of di- and trinucleotides. The genetic variability generated by these markers was evaluated in 51 isolates. The discriminatory power produced by combining the information obtained by the six microsatellites was very high. A total of 57 alleles, which generated 44 genotypes, were found.

Conclusions, significance and impact of the study: The multiple analysis of microsatellites proved to be a powerful and agile tool for analysing the genome of S. cerevisiae populations.

PubMed Disclaimer

Publication types