A novel serine/threonine kinase gene, STK33, on human chromosome 11p15.3

Abstract

Human chromosomal region 11p15 is known to be associated with several diseases including predispositions to develop various tumor types. In search of candidate genes, a novel human kinase gene is described, STK33, which codes for a serine/threonine protein kinase. The gene was discovered by comparative genome analysis of human chromosome 11p15.3 and its orthologous region on distal mouse chromosome 7. Human STK33 gene contains 12 exons as has been determined by the comparison to the full-length transcript amplified from human uterus RNA. Transcripts are found in a variety of tissues in at least two alternatively spliced forms as revealed by reverse transcriptase-polymerase chain reaction, cDNA sequencing and expressed sequence tag clustering. Phylogenetic analysis suggests that STK33 may belong to the calcium/calmodulin-dependent protein kinase group, even though, like several other members of the group, it lacks the calcium/calmodulin binding domain [FASEB J. 9 (1995) 576]. STK33 shows a differential expression in a variety of normal and malignant tissues.