The predicted structure of the calcium-binding component of troponin

Abstract

The structure prediction of the calcium binding component of troponin (TN-C) incorporates the following assumptions: (1) TN-C contains four regions homologous to the calcium binding "EF hand" of parvalbumin. (2) The four EF hands are arranged in two pairs with overall symmetry, 222. (3) The regions of the calcium binding component of troponin which are not in the four EF hands connect the hands within each pair, one to two and three to four, and connect the pairs, region two to region three. In the resulting model there is a well-defined hydrophobic core made from side chains of all eight helical regions and of the four calcium binding loops. The Ca2+ within pairs are separated by 11 A; while the pairs of Ca2+ are separated from one another by over 30 A. Cys-98 and Tyr-109 are suggested to be sensitive spectroscopic probes. Calcium(1) is suggested to be solvent accessible and most readily replaced by a lanthanide. Because of the overall symmetry of the calcium binding component of troponin, one can anticipate that the inhibitory- and the tropomyosin binding components of troponin are similar to one another.