Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Jan 1;30(1):166-8.
doi: 10.1093/nar/30.1.166.

HUGE: a database for human large proteins identified in the Kazusa cDNA sequencing project

Reiko Kikuno  1 , Takahiro Nagase, Mina Waki, Osamu Ohara
Affiliations

HUGE: a database for human large proteins identified in the Kazusa cDNA sequencing project

Reiko Kikuno et al. Nucleic Acids Res. .

Abstract

We have been developing a HUGE database to summarize results from the sequence analysis of human novel large (>4 kb) cDNAs identified in the Kazusa cDNA sequencing project, systematically designated KIAA plus a four-digit number. HUGE currently contains nearly 2000 gene/protein characteristic tables harboring the results of the computer-assisted analysis of the cDNA and the predicted protein sequences together with those of expression profiling and chromosomal mapping. In the updated version of HUGE, we made it possible to compare each KIAA cDNA sequence with the corresponding entry in the human draft genome sequence that was published recently. Approximately 90% of KIAA cDNAs in HUGE can be localized along the human genome for at least half or more of the cDNA's length. Any nucleotide differences between the cDNA and the corresponding genomic sequences are also presented in detail. This new version of HUGE greatly helps us evaluate the completeness of cDNA clones and the accuracy of cDNA/genomic sequences. More interestingly, in some cases, the ability to compare cDNA with genomic sequences allows us to identify candidate sites of RNA editing. HUGE is available on the World Wide Web at http://www.kazusa.or.jp/huge.

PubMed Disclaimer

References

    1. Ohara O., Nagase,T., Ishikawa,K.-I., Nakajima,D., Ohira,M., Seki,N. and Nomura,N. (1997) Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins. DNA Res., 4, 53–59. - PubMed
    1. Nagase T., Nakayama,M., Nakajima,D., Kikuno,R. and Ohara,O. (2001) Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res., 8, 85–95. - PubMed
    1. Kikuno R., Nagase,T., Suyama,M., Waki,M., Hirosawa,M. and Ohara,O. (2000) HUGE: a database for human large proteins identified in the Kazusa cDNA sequencing project. Nucleic Acids Res., 28, 331–332. - PMC - PubMed
    1. Hirosawa M., Isono,K., Hayes,W. and Borodovsky,M. (1997) Gene identification and classification in the Synechocystis genomic sequence by recursive gene mark analysis. DNA Seq., 8, 17–29 - PubMed
    1. International Human Genome Sequencing Consortium (2001) Initial sequencing and analysis of the human genome. Nature, 409, 860–921. - PubMed

Publication types