Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Jan 1;30(1):416-7.
doi: 10.1093/nar/30.1.416.

RIDOM: Ribosomal Differentiation of Medical Micro-organisms Database

Dag Harmsen  1 , Jörg Rothgänger, Matthias Frosch, Jürgen Albert
Affiliations

RIDOM: Ribosomal Differentiation of Medical Micro-organisms Database

Dag Harmsen et al. Nucleic Acids Res. .

Abstract

The ribosomal differentiation of medical micro-organisms (RIDOM) web server, first described by Harmsen et al. [Harmsden,D., Rothganger,J., Singer,C., Albert,J. and Frosch,M. (1999) Lancet, 353, 291], is an evolving electronic resource designed to provide micro-organism differentiation services for medical identification needs. The diagnostic procedure begins with a specimen partial small subunit ribosomal DNA (16S rDNA) sequence. Resulting from a similarity search, a species or genus name for the specimen in question will be returned. Where the first results are ambiguous or do not define to species level, hints for further molecular, i.e. internal transcribed spacer, and conventional phenotypic differentiation will be offered ('sequential and polyphasic approach'). Additionally, each entry in RIDOM contains detailed medical and taxonomic information linked, context-sensitive, to external World Wide Web services. Nearly all sequences are newly determined and the sequence chromatograms are available for intersubjective quality control. Similarity searches are now also possible by direct submission of trace files (ABI or SCF format). Based on the PHRED/PHRAP software, error probability measures are attached to each predicted nucleotide base and visualised with a new 'Trace Editor'. The RIDOM web site is directly accessible on the World Wide Web at http://www.ridom.de/. The email address for questions and comments is webmaster@ridom.de.

PubMed Disclaimer

References

    1. Fredricks D.N. and Relman,D.A. (1996) Sequence-based identification of microbial pathogens: a reconsideration of Koch’s postulates. Clin. Microbiol. Rev., 9, 18–33. - PMC - PubMed
    1. Harmsen D., Heesemann,J., Brabletz,T., Kirchner,T. and Müller-Hermelink,H.K. (1994) Heterogeneity among Whipple’s-disease-associated bacteria. Lancet, 343, 1288. - PubMed
    1. Benson D.A., Karsch-Mizrachi,I., Lipman,D.J., Ostell,J., Rapp,B.A. and Wheeler,D.L. (2000) GenBank. Nucleic Acids Res., 28, 15–18. Updated article in this issue: Nucleic Acids Res. (2002), 30, 17–20. - PMC - PubMed
    1. Maidak B.L., Cole,J.R., Lilburn,T.G., Parker,C.T.,Jr, Saxman,P.R., Farris,R.J., Garrity,G.M., Olsen,G.J., Schmidt,T.M. and Tiedje,J.M. (2001) The RDP-II (Ribosomal Database Project). Nucleic Acids Res., 29, 173–174. - PMC - PubMed
    1. Harmsen D., Rothgänger,J., Singer,C., Albert,J. and Frosch,M. (1999) Intuitive hypertext-based molecular identification of micro-organisms. Lancet, 353, 291. - PubMed