Selected fundamental aspects of chiral electromigration techniques and their application to pharmaceutical and biomedical analysis

Abstract

While capillary electrophoresis has been established as a major enantioseparation technique within the last decade, the potential of capillary electrochromatography is still studied extensively. This review summarizes recent applications of electromigration techniques with regard to the enantioseparation of chiral drugs. The first part discusses the general aspects of migration models and the enantiomer migration order. The application of capillary electrophoresis to chiral pharmaceutical analysis considers recent literature on: (1) chiral resolutions of non-racemic mixtures of enantiomers for the development of assays and the determination of the stereochemical purity of the drugs, (2) chiral separations of compounds in pharmaceutical formulations and products, and (3) enantioseparations of drugs in biological samples. A shorter section devoted to chiral electrochromatography discusses some fundamental aspects as well as the application to the chiral analysis of drugs including bioanalysis.