Extensive chromosome aberrations caused by [3H]thymidine incorporation in a diploid monkey cell line DBS-FRhL-2

Abstract

Extensive chromosome aberrations were induced in a diploid monkey cell line designated as DBS-FRhL-2 after exposure to [3H]thymidine ([3H]Tdr) for either 30 or 60 min at a dose of 1 muCi per ml of medium. Cultures exposed to [3H]Tdr for a longer period had significantly larger numbers of aberrations than those exposed for a shorter period. The most common type of aberrations were chromatid breaks. The majority of aberrations were observed in cells which were in contact with [3H]Tdr during S phase, especially the middle S. Cells from cultures of early and late passages exposed to [3H]Tdr were affected to a similar extent when chromosomes were examined. No clear relationship between sites of breakage and intensity of labeling could be established.