Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Sep;7(5):419-26.
doi: 10.1007/BF01003879.

A quantitative microspectrophotometric study of the lead precipitation reaction for the histochemical demonstration of acid phosphatase

R L Cabrini, A C Frasch, M E Itoiz

A quantitative microspectrophotometric study of the lead precipitation reaction for the histochemical demonstration of acid phosphatase

R L Cabrini et al. Histochem J. 1975 Sep.

Abstract

The possibility of quantitating microspectrophotometrically the lead precipitation reaction for the histochemical demonstration of acid phosphatase was studied using the following models: sections of rat kidney and human prostate gland, and polyacrylamide films with incorporated prostate gland homogenate. The activity of the polyacrylamide models was previously evaluated by a biochemical method and the specificity of the reaction was controlled with specific inhibitors. Linear relations between cytophotometric reading and enzymatic activity, and between optical densities and different incubation periods, were demonstrated. The results obtained indicate the validity of the microspectrophotometric quantitation for this reaction with direct readings over a wide range of the visible light spectrum.

PubMed Disclaimer

References

    1. J Histochem Cytochem. 1967 Aug;15(11):631-45 - PubMed
    1. Histochem J. 1970 Jul;2(4):297-313 - PubMed
    1. J Periodontal Res. 1972;7(1):14-20 - PubMed
    1. Physiol Zool. 1950 Jul;23(3):169-98 - PubMed
    1. J Gen Physiol. 1949 Nov;33(2):125-46 - PubMed