Cellular screening assays using fluorescence microscopy

Abstract

The recent development of automated fluorescence imaging systems has enabled fluorescence microscopy to be used for the purposes of compound screening. This information-rich technique has found various applications, including screening for the effect of kinase inhibitors on the cytoskeleton, agonist-stimulated receptor internalization, and protein phosphorylation and acetylation. The discovery of novel fluorescent proteins and new fluorescent dyes will find many applications in multichannel fluorescence imaging assays.