[Isolation and immunochemical study of the toxic complex of Cl. botulinum type F]
[Article in
Russian]
- PMID: 117654
Item in Clipboard
[Isolation and immunochemical study of the toxic complex of Cl. botulinum type F]
[Article in
Russian]
Zh Mikrobiol Epidemiol Immunobiol.
1979 Dec.
Abstract
The toxic comples of Cl. botulinum, type F, was separated into the toxic and nontoxic protein fractions by the methods of ion exchange chromatography and gel filtration in accordance with a specially devised purification scheme. Highly purified, electrophoretically and serologically homogeneous toxin with a molecular weight of 150,000 and potency equal to 10 X 10(6) DLM per 1 mg of protein was isolated from the toxic fraction. The nontoxic protein component had faintly pronounced hemagglutinating properties and was essentially different from type A and B hemagglutinins. The toxic complex of Cl. botulinum, type F, was shown to contain a proteolytically active fraction.
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