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. 1975 Jan;1(1):96-101.
doi: 10.1128/jcm.1.1.96-101.1975.

Purification of Japanese encephalitis virus vaccine by zonal centrifugation

K Okuda, K Ito, K Miyake, M Morita, M Ogonuki

Purification of Japanese encephalitis virus vaccine by zonal centrifugation

K Okuda et al. J Clin Microbiol. 1975 Jan.

Abstract

A large-scale procedure for purification and concentration of Japanese encephalitis virus vaccine by a continuous-flow isopycnic banding technique in a sucrose density gradient solution, using a K-III zonal centrifuge rotor, is presented. The quality of zonal-purified vaccine was compared with commercial and Japanese National Institutes of Health reference vaccines for antigenicity, immunodiffusion, and allergic encephalitogenicity tests to show its high purity.

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