Microchromatography of hemoglobins. II. A simplified procedure for the determination of hemoglobin A2
- PMID: 1176817
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Microchromatography of hemoglobins. II. A simplified procedure for the determination of hemoglobin A2
J Lab Clin Med.
1975 Oct.
Abstract
A modification of the microchromatographic procedure for Hb-A2 which utilizes DEAE-cellulose (Reference 1) allows the quantitation of Hb-A2 without interference from any Hb-S in the sample. Elution of Hb-A2 with a glycine-KCN developer is much less sensitive to minor change in pH of the developer, but is greatly dependent on the pH of the ion exchanger.
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