A simplified procedure for synthesis of di-[14C]acyl-labeled lecithins
- PMID: 1176834
Item in Clipboard
A simplified procedure for synthesis of di-[14C]acyl-labeled lecithins
J Lipid Res.
1975 Sep.
Free article
Abstract
A simplified procedure for synthesis of 1,2-di-[1'-14C]oleoyl-, 1,2-di-[1'-14C]linoleoyl-, and 1,2-di-[1'-14C]eicosatrienoyl-sn-glycero-3-phosphorylcholine is described. The method involves acylation of the CdCl2 complex of glycerophosphorylcholine with a 14C-labeled fatty acid in the presence of trifluoracetic anhydride and pyridine. The 14C-labeled lecithin is isolated in pure form by preparative thin-layer chromatography and alumina column chromatography in an overall yield of 12-24%. No isomerization or peroxidation of the unsaturated acids was detected.
Similar articles
-
An improved method for the preparation of unsaturated phosphatidylcholines: acylation of sn-glycero-3-phosphorylcholine in the presence of sodium methylsulfinylmethide.J Lipid Res. 1977 Jul;18(4):548-52. J Lipid Res. 1977. PMID: 894147
-
Direct desaturation of eicosatrienoyl lecithin to arachidonoyl lecithin by rat liver microsomes.J Biol Chem. 1977 Jan 10;252(1):68-73. J Biol Chem. 1977. PMID: 833130
-
Preparation of labeled alkyl acyl glycerophosphorylcholine.J Lipid Res. 1970 Sep;11(5):496-8. J Lipid Res. 1970. PMID: 5533670
-
Chemical synthesis of choline-labeled lecithins and sphingomyelins.Methods Enzymol. 1975;35:533-41. doi: 10.1016/0076-6879(75)35181-1. Methods Enzymol. 1975. PMID: 164606 No abstract available.
-
New trends in ganglioside chemistry.Adv Exp Med Biol. 1988;228:437-64. doi: 10.1007/978-1-4613-1663-3_16. Adv Exp Med Biol. 1988. PMID: 3051920 Review.
Cited by
-
Membrane-bound phospholipid desaturases.Lipids. 1979 Feb;14(2):159-65. doi: 10.1007/BF02533867. Lipids. 1979. PMID: 218072
-
A review on methods of phospholipase A determination.Agents Actions. 1979 Oct;9(4):382-9. doi: 10.1007/BF01970666. Agents Actions. 1979. PMID: 42289 Review.
-
Glycerophospholipid synthesis: improved general method and new analogs containing photoactivable groups.Proc Natl Acad Sci U S A. 1977 Oct;74(10):4315-9. doi: 10.1073/pnas.74.10.4315. Proc Natl Acad Sci U S A. 1977. PMID: 270675 Free PMC article.
-
An improved procedure for the synthesis of choline phospholipids via 2-bromoethyl dichlorophosphate.Lipids. 1982 Jun;17(6):453-9. doi: 10.1007/BF02535226. Lipids. 1982. PMID: 27519713
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
