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. 2002 Jan;68(1):65-72.
doi: 10.1128/AEM.68.1.65-72.2002.

Characterization of Pseudomonas spp. associated with spoilage of gilt-head sea bream stored under various conditions

P Tryfinopoulou  1 , E Tsakalidou, G-J E Nychas
Affiliations

Characterization of Pseudomonas spp. associated with spoilage of gilt-head sea bream stored under various conditions

P Tryfinopoulou et al. Appl Environ Microbiol. 2002 Jan.

Abstract

The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (Sparus aurata) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 Pseudomonas strains isolated from S. aurata stored under different temperatures (at 0, 10, and 20 degrees C) and packaging conditions (air and a modified atmosphere of 40% CO(2)-30% N(2)-30% O(2)). Pseudomonas lundensis was the predominant species, followed by Pseudomonas fluorescens, while Pseudomonas fragi and Pseudomonas putida were detected less frequently. Fluorescent Pseudomonas strains dominated under air conditions, while proteolytic and less lipolytic strains dominated under modified-atmosphere packaging. Different storage conditions appear to govern the selection of pseudomonads in gilt-head sea bream fish.

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Figures

FIG. 1.
FIG. 1.
Changes in the Pseudomonas population of naturally spoiled gilt-head sea bream fish during storage in air (a) and with MAP (b) at 0°C (▪), 10°C (•), and 20°C (▴), as fitted with the Baranyi model.
FIG. 2.
FIG. 2.
Clustering of protein electropherograms of 115 strains of Pseudomonas. The Pearson product-moment correlation coefficient and the UPGMA method were used. The horizontal scale represents percent similarities. The vertical scale recognizes the strains and clusters of Pseudomonas strains.
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