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. 2001 Dec;39(4):307-12.
doi: 10.3347/kjp.2001.39.4.307.

Antigenic profile and localization of Clonorchis sinensis proteins in the course of infection

S J Hong  1 , T Y Kim, K Y Song, W M Sohn, S Y Kang
Affiliations

Antigenic profile and localization of Clonorchis sinensis proteins in the course of infection

S J Hong et al. Korean J Parasitol. 2001 Dec.

Abstract

In the course of Clonorchis sinensis infection, antigens presented to the hosts may be in a close relation to growth of the fluke. The antigenic proteins stimulating IgG antibody production were chronologically identified by immunoblot and localized by immunohistochemical staining. In the early stage of infection until 12 weeks post-infection (PI), antigens were proteins with molecular mass larger than 34 kDa which were derived from the tegument, testes and intrauterine eggs. After 20 weeks PI, antigens recognized were 29, 27 and 26 kDa proteins from the intestine, excretory bladder and reproductive organs. It is suggested that the tegumental proteins are the most potent antigens and the excretory-secretory proteins with middle molecular mass of 26-45 kDa contribute to the high level production of antibodies after 20 weeks of the C. sinensis infection.

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Figures

Fig. 1
Fig. 1
An immunoblot of adult Clonorchis sinensis crude antigen against a rabbit serum. The sera were collected from the rabbit over one year after the metacercarial infection.
Figs. 2-7
Figs. 2-7
Immunohistochemical staining of adult Clonorchis sinensis with the rabbit sera of C. sinensis-infection. Fig. 2. Control stained with a pre-infection serum. In, intestine; Tg, tegument; Ts, testes. Fig. 3. A serum of 3-week post-infection (PI) produced light red color in tegument and in uterus (Ut) and eggs. Fig. 4. The intestinal epithelium and excretory bladder (EB) were stained lightly and the tegument was moderately by a serum of 4-week PI. Fig. 5. Stained with a serum of 28-week PI. Note the stains in the vitelline follicles (VF) and wall of seminal receptacle (SR). The mesenchymal tissue was recognized to be a light staining. Fig. 6. The epithelium of miracidium in intra-uterine egg was stained moderate by a serum of 28-week PI. Bar is 50 µm. Fig. 7. A serum of 32-week PI produced a strong stain in the tegument and moderate ones in testes and intestinal epithelium. The mesenchymal tissue was stained by a moderate degree. Bars are 100 µm.
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References

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