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. 2002 Mar;49(1):97-101.
doi: 10.1016/s0167-7012(01)00364-5.

A one-step method for genetic transformation of non-piliated Neisseria meningitidis

John A Bogdan  1 , C A S A Minetti, M S Blake
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A one-step method for genetic transformation of non-piliated Neisseria meningitidis

John A Bogdan et al. J Microbiol Methods. 2002 Mar.

Abstract

A one-step method to chemically transform non-piliated Neisseria meningitidis strains previously resistant to conventional electrochemical transformation procedures has been developed. This method has been used to generate genetically engineered meningococcal strains disrupted in the structural rmpM gene encoding Rmp.

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