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. 2002 Feb 8;295(5557):1089-92.
doi: 10.1126/science.1068228. Epub 2002 Jan 3.

Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning

Liangxue Lai  1 , Donna Kolber-Simonds, Kwang-Wook Park, Hee-Tae Cheong, Julia L Greenstein, Gi-Sun Im, Melissa Samuel, Aaron Bonk, August Rieke, Billy N Day, Clifton N Murphy, David B Carter, Robert J Hawley, Randall S Prather
Affiliations

Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning

Liangxue Lai et al. Science. .

Abstract

The presence of galactose alpha-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the alpha-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.

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