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. 1979 Jun;17(5-6):499-516.
doi: 10.1007/BF00498886.

Increasing the resolution of polyacrylamide gel electrophoresis by varying the degree of gel crosslinking

G Johnson

Increasing the resolution of polyacrylamide gel electrophoresis by varying the degree of gel crosslinking

G Johnson. Biochem Genet. 1979 Jun.

Abstract

Resolution of polyacrylamide gel electrophoresis may be substantially improved by taking advantage of the gel sieving effects of varying concentrations of bisacrylamide crosslinker. A dilution procedure is described which permits simultaneous variation of both total acrylamide concentration and percent crosslinking within a single linear regression alaysis.

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