Identification of female sex pheromone of the rice leaf bug, Trigonotylus caelestialium

Abstract

The female sex pheromone of the rice leaf bug, Trigonotylus caelestialium, was analyzed by GC-EAD and GC-MS. Ten EAD active compounds-n-hexyl n-hexanoate, (E)-2-hexenyl n-hexanoate, n-hexyl (E)-2-hexenoate, n-octyl n-butyrate, n-octyl n-hexanoate, (E)-2-octenyl n-hexanoate, n-pentyl n-hexanoate, n-hexyl n-butyrate, (E)-2-hexenyl n-butyrate, and n-hexyl (E)-2-butenoate-were detected in the ratio of 1,000:414-491:trace-5:5-11:55-71:50-63:trace-3:225:90:32 from female body extracts, and in the ratio of 1,000:271-342:10-43:1-3:58-78:14-19:trace:178:36:26 from male body extracts. Field trapping tests with these synthetic compounds indicated that n-hexyl n-hexanoate, (E)-2-hexenyl n-hexanoate, and n-octyl n-butyrate are pheromone components, and mixtures in ratios of 1,000:400-500:10-100 were more attractive to males. Doses ranging from 4.29 microg to 14.3 microg of the three-component mixture in the ratio of 1,000:400:30 loaded into glass capillary tubes were most attractive to males.