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. 2002 Jan 11;108(1):121-33.
doi: 10.1016/s0092-8674(01)00635-3.

Beta phorbol ester- and diacylglycerol-induced augmentation of transmitter release is mediated by Munc13s and not by PKCs

Jeong Seop Rhee  1 , Andrea Betz, Sonja Pyott, Kerstin Reim, Frederique Varoqueaux, Iris Augustin, Dörte Hesse, Thomas C Südhof, Masami Takahashi, Christian Rosenmund, Nils Brose
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Free article

Beta phorbol ester- and diacylglycerol-induced augmentation of transmitter release is mediated by Munc13s and not by PKCs

Jeong Seop Rhee et al. Cell. .
Free article

Abstract

Munc13-1 is a presynaptic protein with an essential role in synaptic vesicle priming. It contains a diacylglycerol (DAG)/beta phorbol ester binding C(1) domain and is a potential target of the DAG second messenger pathway that may act in parallel with PKCs. Using genetically modified mice that express a DAG/beta phorbol ester binding-deficient Munc13-1(H567K) variant instead of the wild-type protein, we determined the relative contribution of PKCs and Munc13-1 to DAG/beta phorbol ester-dependent regulation of neurotransmitter release. We show that Munc13s are the main presynaptic DAG/beta phorbol ester receptors in hippocampal neurons. Modulation of Munc13-1 activity by second messengers via the DAG/beta phorbol ester binding C(1) domain is essential for use-dependent alterations of synaptic efficacy and survival.

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